Journal: Regenerative Therapy
Article Title: In vitro and in vivo study of concentrated growth factor (CGF) mediating macrophage polarization in bone defect repair
doi: 10.1016/j.reth.2025.04.013
Figure Lengend Snippet: Surface characteristics of C GF and its effect on macrophages. A: CGF and its three-dimensional structure under electron microscopy; The proliferation of BMDM treated with different concentrations of CGF; B: ELISA method is used to detect the secretion of IL-1 β 、TNF- α 、VEGF-A、IL-10 after CGF acts on M0 and M1 macrophages; C: Western blot detection of protein expression of Arg-1 and iNOS after CGF acts on M0 and M1 macrophages; D: Western blot detection of JAK/STAT pathway related protein expression after CGF acts on M0 and M1 macrophages, respectively (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001. #, P < 0.05; ##, P < 0.01; ###, P < 0.001; ####, P < 0.0001. # compared with the control group, ns, P > 0.05,one-way ANOVA).
Article Snippet: BMDM macrophage cell lines (Procell Life Science & Technology Co., Ltd.) were used for in vitro studies.
Techniques: Electron Microscopy, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control